Laser-based Preparation of Biological Tissue

Computational Laser Spectroscopy in a Biological Tissue

We present a numerical spectroscopic study of visible and infrared laser radiation in a biological tissue. We derive a solution of a general two-dimensional time dependent radiative transfer equation in a tissue-like medium. The used model is suitable for many situations especially when the external source is time-dependent or continuous. We use a control volume-discrete ordinate method associa...

Statistical Analysis of Instantaneous Effect of Polarized He-Ne Laser Radiation on the Elastic Modulus of Soft Biological Tissue

Introduction: This work is dedicated to investigating the effect of applying polarized He-Ne laser beam (λ= 632 nm, 10 mW, 1 j/cm2) with polarization direction perpendicular to the stress direction on the tensile strength of biological tissue (elasticity index of the Pleura 10*4*0.1 mm).   Method and Material: The optimized characteristics of the laser illumi...

Thermal effects of laser radiation in biological tissue.

A theoretical model is presented that simulates the thermal effects of laser radiation incident on biological tissue. The multiple scattering and absorption of the laser beam and the thermal diffusion process in the tissue are evaluated by a numerical technique that is well suited for microcomputers. Results are compared with recent empirical observations.

Selective delivery of laser energy to biological tissue based on refractive-index differences.

We report a novel method for selective laser energy delivery into biological tissues based on refractive-index differences. As a specific example, Ho:YAG laser energy is delivered into fat preferentially over other soft tissue, despite the fact that water has a higher absorption coefficient at this wavelength than fat.

Tissue preparation for laser capture microdissection and RNA extraction from fresh frozen breast tissue.